2,43
1,65
1,98
0,73
1,88
1,81
2,43
2.2 Standardsubstanzen, déi an der Kalibrierungskurve vun der relativer Molekularmasseverdeelung benotzt ginn: Insulin, Mykopeptiden, Glycin-Glycin-Tyrosin-Arginin, Glycin-Glycin-Glycin
3 Instrument an Ausrüstung
23.2
21.4
22.2
16.1
22.3
20.8
23,9
27,5
Insgesamt ass den Undeel vun Aminosaieren an de Produkter vu Sustar méi héich wéi dee vun de Produkter vu Zinpro.
Deel 8 Auswierkunge vum Gebrauch
Auswierkunge vu verschiddene Quelle vu Spuerelementer op d'Produktiounsleistung an d'Eeërqualitéit vu Legehennen an der spéider Legeperiod
Produktiounsprozess
Gezielte Chelattechnologie
Schéier-Emulgatiounstechnologie
Drocksprëtz- a Trocknungstechnologie
Kill- a Feuchtigkeitstechnologie
Fortgeschratt Technologie fir d'Ëmweltkontroll
Anhang A: Methoden fir d'Bestimmung vun der relativer molekularer Masseverdeelung vu Peptiden
Adoptioun vum Standard: GB/T 22492-2008
1 Testprinzip:
Et gouf duerch Héichleistungs-Gelfiltratiounschromatographie bestëmmt. Dat heescht, mat Hëllef vun engem porösen Fëllstoff als stationärer Phas, baséiert op dem Ënnerscheed an der relativer Molekularmassgréisst vun de Proufkomponenten fir d'Trennung, detektéiert bei der Peptidbindung vun der ultravioletter Absorptiounswellelängt vun 220 nm, mat Hëllef vun der spezialiséierter Datenveraarbechtungssoftware fir d'Bestimmung vun der relativer Molekularmassverdeelung duerch Gelfiltratiounschromatographie (dh der GPC-Software), goufen d'Chromatogrammer an hir Donnéeën veraarbecht, berechent fir d'Gréisst vun der relativer Molekularmass vum Sojabounenpeptid an de Verdeelungsberäich ze kréien.
2. Reagenzien
D'Experimentalwaasser soll der Spezifikatioun vum Sekundärwaasser am GB/T6682 entspriechen, d'Benotzung vu Reagenzien, ausser fir speziell Bestëmmungen, ass analytesch reng.
2.1 Zu de Reagenzien gehéieren Acetonitril (chromatographesch reng), Trifluoressigsäure (chromatographesch reng),
2.2 Standardsubstanzen, déi an der Kalibrierungskurve vun der relativer Molekularmasseverdeelung benotzt ginn: Insulin, Mykopeptiden, Glycin-Glycin-Tyrosin-Arginin, Glycin-Glycin-Glycin
3 Instrument an Ausrüstung
3.1 Héichleistungsflëssegchromatographie (HPLC): eng chromatographesch Aarbechtsstatioun oder Integrator mat engem UV-Detektor a GPC-Datenveraarbechtungssoftware.
3.2 Mobilphasen-Vakuumfiltratiouns- an Entgasungseenheet.
3.3 Elektronesch Balance: graduéierte Wäert 0,000 1g.
4 Betriebsschrëtt
4.1 Chromatographesch Konditiounen an Systemadaptatiounsexperimenter (Referenzbedingungen)
- 4.1.1 Chromatographesch Kolonn: TSKgelG2000swxl300 mm×7,8 mm (bannenduerchmiesser) oder aner Gelkolonnen vum selwechten Typ mat ähnlecher Leeschtung, déi fir d'Bestimmung vu Proteinen a Peptiden gëeegent sinn.
- 4.1.2 Mobil Phas: Acetonitril + Waasser + Trifluoressigsäure = 20 + 80 + 0,1.
- 4.1.3 Detektiounswellelängt: 220 nm.
- 4.1.4 Duerchflussrate: 0,5 ml/min.
- 4.1.5 Detektiounszäit: 30 Minutten.
- 4.1.6 Proufinjektiounsvolumen: 20μL.
- 4.1.7 Kolonnentemperatur: Raumtemperatur.
- 4.1.8 Fir datt de chromatographesche System den Detektiounsufuerderunge gerecht gëtt, gouf festgeluecht, datt ënner den uewe genannten chromatographesche Konditiounen d'Effizienz vun der Gelchromatographie-Sail, also déi theoretesch Zuel vun de Placken (N), net manner wéi 10000 ass, berechent op Basis vun de Peaks vum Tripeptidstandard (Glycin-Glycin-Glycin).
- 4.2 Erstellung vu Standardkurven fir relativ Molekularmass
- Déi uewe genannten ënnerschiddlech relativ molekular Mass-Peptid-Standardléisungen mat enger Massekonzentratioun vun 1 mg/ml goufen duerch mobil Phasenanpassung virbereet, an engem bestëmmte Verhältnis gemëscht an duerno duerch eng organesch Phasenmembran mat enger Poregréisst vun 0,2 μm~0,5 μm gefiltert an an d'Prouf injizéiert, an duerno goufen d'Chromatogrammer vun de Standarden kritt. D'Kalibratiounskurve fir relativ molekular Mass an hir Equatioune goufen duerch d'Analyse vum Logarithmus vun der relativer molekularer Mass géint d'Retentiounszäit oder duerch linear Regressioun kritt.
4.3 Proufbehandlung
Weet genee 10 mg vun der Prouf an engem 10 ml Moosskolben of, füügt e bësse mobil Phas derbäi, schëddelt 10 Minutten mat Ultraschall, sou datt d'Prouf komplett opgeléist a gemëscht ass, verdënnt mat der mobiler Phas op d'Waag, an dann duerch eng Membran vun der organescher Phas mat enger Poregréisst vun 0,2 μm~0,5 μm gefiltert, an de Filtrat gouf no de chromatographesche Konditiounen an A.4.1 analyséiert.
- 5. Berechnung vun der relativer molekularer Masseverdeelung
- Nodeems d'Proufléisung, déi a 4.3 ënner de chromatographesche Konditioune vu 4.1 preparéiert gouf, analyséiert gouf, kann déi relativ Molekularmass vun der Prouf an hire Verdeelungsberäich erausfonnt ginn, andeems d'chromatographesch Donnéeë vun der Prouf an d'Kalibratiounskurve 4.2 mat GPC-Datenveraarbechtungssoftware agesat ginn. D'Verdeelung vun de relative Molekularmassen vun den ënnerschiddleche Peptiden kann mat der Peak-Flächennormaliséierungsmethod berechent ginn, no der Formel: X=A/A total×100
- An der Formel: X - De Massenundeel vun engem relative molekulare Peptid am gesamte Peptid an der Prouf, %;
- A - Peakfläch vun engem relative molekulare Massepeptid;
- Total A - d'Zomm vun de Peakflächen vun all relativer molekulare Massepeptid, berechent op eng Dezimalplaz.
- 6 Widderhuelbarkeet
- Den absoluten Ënnerscheed tëscht zwou onofhängege Bestëmmungen, déi ënner Widderhuelbarkeetsbedingunge kritt goufen, däerf net méi wéi 15% vum arithmetesche Mëttelwäert vun den zwou Bestëmmunge sinn.
- Anhang B: Methoden fir d'Bestimmung vu fräien Aminosaieren
- Iwwernahm vum Standard: Q/320205 KAVN05-2016
- 1.2 Reagenzien a Materialien
- Äiseddik: analytesch reng
- Perchlorsäure: 0,0500 mol/L
- Indikator: 0,1% Kristallviolettindikator (Äisessig)
- 2. Bestëmmung vu fräien Aminosaieren
D'Prouwen goufen 1 Stonn bei 80°C gedréchent.
Leet d'Prouf an en dréchenen Behälter, fir datt se natierlech op Raumtemperatur ofkille kann oder op eng brauchbar Temperatur ofkille kann.Wee ongeféier 0,1 g vun der Prouf (genau op 0,001 g) an en dréchene Konischen Kolben vun 250 ml of.Gitt séier zum nächste Schrëtt weider, fir ze verhënneren, datt d'Prouf d'Ëmweltfiichtegkeet absorbéiert.Gidd 25 ml Äisesseg derbäi a réiert gutt fir net méi wéi 5 Minutten.Füügt 2 Drëpse Kristallviolettindikator derbäiTitréiert mat 0,0500 mol / L (±0,001) Standardtitratiounsléisung vu Perchlorsäure, bis d'Léisung vu violett bis zum Endpunkt ännert.
Schreift de verbrauchte Volumen vun der Standardléisung op.
- Maacht gläichzäiteg den Test mat engem Blankett.
- 3. Berechnung a Resultater
- Den Inhalt vun fräien Aminosäuren X am Reagens gëtt als Massenundeel (%) ausgedréckt a gëtt no der Formel berechent: X = C × (V1-V0) × 0,1445/M × 100%, an der Formel:
- C - Konzentratioun vun der Standardperchlorsäureléisung a Mol pro Liter (mol/L)
- V1 - Volumen, dat fir d'Titratioun vu Prouwe mat Standardperchlorsäureléisung benotzt gëtt, a Milliliter (mL).
- Vo - Volumen, dat fir d'Titratiounsblindprouf mat Standardperchlorsäureléisung benotzt gëtt, a Milliliter (mL);
M - Mass vun der Prouf, a Gramm (g).
| 0,1445: Duerchschnëttlech Mass vun Aminosaieren, déi gläichwäerteg mat 1,00 mL Standard-Perchlorsäureléisung ass [c(HClO4) = 1,000 mol/L]. | 4.2.3 Ceriumsulfat Standardtitratiounsléisung: Konzentratioun c [Ce (SO4) 2] = 0,1 mol/L, preparéiert no GB/T601. | |
| Adoptioun vun de Standarden: Q/70920556 71-2024 | 1. Bestëmmungsprinzip (Fe als Beispill) | Aminosaier-Eisen-Komplexe hunn eng ganz niddreg Léislechkeet an wasserfreiem Ethanol a fräi Metallionen si léislech an wasserfreiem Ethanol. Den Ënnerscheed an der Léislechkeet tëscht deenen zwee an wasserfreiem Ethanol gouf benotzt fir d'Chelatiounsquote vun Aminosaier-Eisen-Komplexe ze bestëmmen. |
| An der Formel: V1 - Volumen vun der Ceriumsulfat-Standardléisung, déi fir d'Titratioun vun der Testléisung verbraucht gouf, mL; | Waasserfräien Ethanol; de Rescht ass dee selwechte wéi an der Klausel 4.5.2 am GB/T 27983-2011. | 3. Schrëtt vun der Analyse |
| Maacht zwou parallel Versich. Weet 0,1 g vun der Prouf of, déi 1 Stonn bei 103 ± 2 ℃ gedréchent gouf, mat enger Genauegkeet vun 0,0001 g, füügt 100 ml wasserfreien Ethanol derbäi fir se opzeléisen, filtert, filtert de Rescht, wäscht en op d'mannst dräimol mat 100 ml wasserfreien Ethanol, da gitt de Rescht an e 250 ml Kegelkolben, füügt 10 ml Schwefelsäureléisung no Klausel 4.5.3 am GB/T27983-2011 derbäi, a maacht dann déi folgend Schrëtt no Klausel 4.5.3 "Heat to dissolve and then let cool" am GB/T27983-2011. Maacht gläichzäiteg den Blanktest. | 4. Bestëmmung vum Gesamteisengehalt | 4.1 De Prinzip vun der Bestëmmung ass dee selwechte wéi an der Klausel 4.4.1 am GB/T 21996-2008. |
4.2. Reagenzien & Léisungen
| 4.2.1 Gemëschte Säure: Füügt 150 ml Schwefelsäure an 150 ml Phosphorsäure zu 700 ml Waasser bäi a vermëscht gutt. | 4.2.2 Natriumdiphenylaminsulfonat-Indikatorléisung: 5g/L, preparéiert no GB/T603. | 4.2.3 Ceriumsulfat Standardtitratiounsléisung: Konzentratioun c [Ce (SO4) 2] = 0,1 mol/L, preparéiert no GB/T601. | |
| 4.3 Schrëtt vun der Analyse | Maacht zwou Versich parallel. Weet 0,1 g vun der Prouf of, mat enger Genauegkeet vun 020001 g, gitt se an e 250 ml Erlenkfläsch, füügt 10 ml gemëschte Säure derbäi, nodeems se opgeléist ass, füügt 30 ml Waasser a 4 Drëpse Natriumdianilinsulfonat-Indikatorléisung derbäi, a maacht dann déi folgend Schrëtt no Klausel 4.4.2 am GB/T21996-2008. Maacht gläichzäiteg den Blanktest. | 4.4 Representatioun vun de Resultater | Den gesamten Eisengehalt X1 vun den Aminosäure-Eisenkomplexer a Bezuch op de Masseundeel vum Eisen, de Wäert ausgedréckt a %, gouf no der Formel (1) berechent: |
| X1=(V-V0)×C×M×10-3×100 | V0 - Ceriumsulfat-Standardléisung, déi fir d'Titratioun vun der Blindléisung verbraucht gouf, mL; | V0 - Ceriumsulfat-Standardléisung, déi fir d'Titratioun vun der Blindléisung verbraucht gouf, mL; | C - Tatsächlech Konzentratioun vun der Ceriumsulfat-Standardléisung, mol/L5. Berechnung vum Eisengehalt a ChelatenDen Eisengehalt X2 am Chelat a Bezuch op de Massefraktioun vum Eisen, de Wäert ausgedréckt a %, gouf no der Formel berechent: x2 = ((V1-V2) × C × 0,05585)/m1 × 100 |
| An der Formel: V1 - Volumen vun der Ceriumsulfat-Standardléisung, déi fir d'Titratioun vun der Testléisung verbraucht gouf, mL; | V2 - Ceriumsulfat-Standardléisung, déi fir d'Titratioun vun der Blindléisung verbraucht gouf, mL;nom1 - Mass vun der Prouf, g. Huelt den arithmetesche Mëttelwäert vun de parallele Bestëmmungsresultater als Bestëmmungsresultater, an den absoluten Ënnerscheed vun de parallele Bestëmmungsresultater ass net méi wéi 0,3%. | 0,05585 - Mass vun Eisen(II), ausgedréckt a Gramm, gläichwäerteg mat 1,00 mL Ceriumsulfat-Standardléisung C[Ce(SO4)2.4H20] = 1,000 mol/L.nom1 - Mass vun der Prouf, g. Huelt den arithmetesche Mëttelwäert vun de parallele Bestëmmungsresultater als Bestëmmungsresultater, an den absoluten Ënnerscheed vun de parallele Bestëmmungsresultater ass net méi wéi 0,3%. | 6. Berechnung vun der ChelatiounsquoteChelatiounsquote X3, de Wäert ausgedréckt a %, X3 = X2/X1 × 100Anhang C: Methoden fir d'Bestimmung vun der Chelatiounsquote vu Zinpro |
Iwwernahm vun der Norm: Q/320205 KAVNO7-2016
1. Reagenzien a Materialien
a) Äiseddik: analytesch reng; b) Perchlorsäure: 0,0500 mol/L; c) Indikator: 0,1% Kristallviolett-Indikator (Äiseddik)
2. Bestëmmung vu fräien Aminosaieren
2.1 D'Prouwen goufen 1 Stonn bei 80°C gedréchent.
2.2 Leet d'Prouf an en dréchene Behälter, fir datt se natierlech op Raumtemperatur ofkille kann oder op eng brauchbar Temperatur ofkille kann.
2.3 Wee ongeféier 0,1 g vun der Prouf (genau op 0,001 g) an en dréchene Konusfläsch vun 250 ml of.
2.4 Gitt séier zum nächste Schrëtt weider, fir ze verhënneren, datt d'Prouf d'Ëmfeldfiichtegkeet absorbéiert.
2.5 Gidd 25 ml Äisesseg derbäi a vermëscht gutt fir net méi wéi 5 Minutten.
2.6 Füügt 2 Drëpse Kristallviolettindikator derbäi.
2.7 Titréiert mat 0,0500 mol/L (±0,001) Standardtitratiounsléisung vu Perchlorsäure, bis d'Léisung fir 15 Sekonnen vu violett op gréng ännert, ouni datt d'Faarf als Endpunkt ännert.
2.8 Schreift de verbrauchte Volumen vun der Standardléisung op.
2.9 Féiert gläichzäiteg den Blanktest duerch.
- 3. Berechnung a Resultater
- katalanesch
- Physicochemical parameters
V1 - Volumen, dat fir d'Titratioun vu Prouwe mat Standardperchlorsäureléisung benotzt gëtt, a Milliliter (mL).
Vo - Volumen, dat fir d'Titratiounsblindprouf mat Standardperchlorsäureléisung benotzt gëtt, a Milliliter (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
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Telefon: 86-18880477902
Produkter
Anorganesch Spuerelementer
- Organesch Spuerelementer
- Swahili
- Personaliséierte Service
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Firmenprofil
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Klickt fir eng Ufro | © Copyright - 2010-2025: All Rechter reservéiert. | Sitemap TOP SICH Telefon |
| Tel. | 86-18880477902 | Javanesesch | E-Mail |
| 8618880477902 | Chineesesch | Franséisch | |
| Bird | Chineesesch | Franséisch | Däitsch Spuenesch |
| Aquatic animals | Japanesch | Koreanesch | Arabesch Griichesch |
| Tierkesch | Italienesch | ||
| Ruminant animal g/head day | January 0.75 | Indonesesch Afrikaans Schweedesch |
Polnesch
- Baskesch
- katalanesch
- Physicochemical parameters
Hindi
laotesch
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Bulgarescg
- Cebuano
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Kroatesch
Hollänesch
| Application object | Urdu Vietnamesesch | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Haitianesch | Haussa | Kinyarwanda Hmong Ungaresch |
| Piglets and fattening pigs | Igbo | Javanesesch | Kannada Khmer kurdesch |
| kirgisesch | laténgesch | ||
| Bird | 300～400 | 45～60 | Mazedonesch Malaiesch Malajalam |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Norweegesch
- pashtu
- Appearance: brownish-yellow granules
- Physicochemical parameters
serbesch
Sesotho
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Sindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Swahili
Tadschikesch
Tamil
Telugu
Thai
| Application object | Urdu Vietnamesesch | Content in full-value feed (mg/kg) | Efficacy |
| Jiddesch | Yoruba | Zuluen | Kinyarwanda Oriya turkmenesch |
| uiguresch | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1,52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
